Hotspot

ycf1

SSC · 133486-139767 · 6282 bp

Pi 0.0186

MarkerSeek score 55.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6282
variable_sites: 234
pi: 0.0186
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCCGGATCAAAAGATTCCT	AGAAAACCGTCACACATGCT	50.0	45.0	57.9	58.0	666-876 bp	1.0	88.2
ycf1_p2	GCCCGGATCAAAAGATTCCT	GAAAACCGTCACACATGCTG	50.0	50.0	57.9	58.0	665-875 bp	1.0	88.2
ycf1_p3	GCCCGGATCAAAAGATTCCT	CCGTCACACATGCTGACTAA	50.0	50.0	57.9	57.9	660-870 bp	1.0	87.9
ycf1_p4	GCCCGGATCAAAAGATTCCT	AGCAACAACCGGCTTTCTTA	50.0	45.0	57.9	58.0	1258-1468 bp	1.0	84.7
ycf1_p5	GCCCGGATCAAAAGATTCCT	GACTGCCGGAAATTCAATGC	50.0	50.0	57.9	58.1	1075-1111 bp	0.8	76.6