Hotspot

clpP1

LSC · 77593-80542 · 2950 bp

Pi 0.0225

MarkerSeek score 58.30

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2950
variable_sites: 119
pi: 0.0225
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TGACCCTGTTAGTCCGTTCT	AAGCAACGGAGCCATCATAG	50.0	50.0	58.0	58.0	1352-1841 bp	1.0	88.6
clpP1_p2	TGACCCTGTTAGTCCGTTCT	CTCGAATCAACTTGCGGGTA	50.0	50.0	58.0	58.0	1993-2479 bp	1.0	88.1
clpP1_p3	TGACCCTGTTAGTCCGTTCT	CACTAGCCATGGGATTGACC	50.0	55.0	58.0	58.0	1844-2330 bp	1.0	88.1
clpP1_p4	CCTCTATACACCGGAGCTCA	CTCGAATCAACTTGCGGGTA	55.0	50.0	58.0	58.0	2155-2641 bp	1.0	87.7
clpP1_p5	CCTCTATACACCGGAGCTCA	CACTAGCCATGGGATTGACC	55.0	55.0	58.0	58.0	2006-2492 bp	1.0	87.7