Hotspot

ycf1-trnN-GUU

SSC · 139768-140508 · 741 bp

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Pi 0.0599
MarkerSeek score 77.30
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
SSC
length_bp
741
variable_sites
108
pi
0.0599
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 TAAGAAAGCCGGTTGTTGCT GGGTTACCTGCTCTTAACGG 45.0 55.0 58.0 58.0 1103-1294 bp 1.0 91.6
ycf1-trnN-GUU_p2 TAAGAAAGCCGGTTGTTGCT TCGGGAGAATCGATGACTGA 45.0 50.0 58.0 57.9 1312-1503 bp 1.0 90.2
ycf1-trnN-GUU_p3 TAAGAAAGCCGGTTGTTGCT GGCAGAATAGCAGAGCAAGT 45.0 50.0 58.0 58.0 1400-1591 bp 1.0 90.0
ycf1-trnN-GUU_p4 TAAGAAAGCCGGTTGTTGCT TAATGTGTTTGCTCGCGGTA 45.0 45.0 58.0 57.9 1343-1534 bp 1.0 89.8
ycf1-trnN-GUU_p5 GCATTGAATTTCCGGCAGTC GGGTTACCTGCTCTTAACGG 50.0 55.0 58.1 58.0 1288-1477 bp 0.8 72.0