Hotspot

ndhF-rpl32

SSC · 121736-122637 · 902 bp

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Pi 0.00177
MarkerSeek score 63.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
902
variable_sites
4
pi
0.00177
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCCGTCGGAAAAAGTA TGAAAAAGCTTTCAACGCCG 45.0 45.0 58.0 58.2 1080-1101 bp 1.0 80.5
ndhF-rpl32_p2 TTGGAAGTGGCACAAAAGGT TGAAAAAGCTTTCAACGCCG 45.0 45.0 58.1 58.2 1033-1054 bp 1.0 79.9
ndhF-rpl32_p3 TGTTGCCGTCGGAAAAAGTA GCCGCCCAATATCCTTTCTT 45.0 50.0 58.0 58.3 1064-1085 bp 1.0 79.4
ndhF-rpl32_p4 TTTGTTGCCGTCGGAAAAAG TGAAAAAGCTTTCAACGCCG 45.0 45.0 57.8 58.2 1082-1103 bp 1.0 79.4
ndhF-rpl32_p5 TTGGAAGTGGCACAAAAGGT GCCGCCCAATATCCTTTCTT 45.0 50.0 58.1 58.3 1017-1038 bp 1.0 78.8