Hotspot

trnN-GUU-ndhF

SSC · 118910-119485 · 576 bp

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Pi 0.0385
MarkerSeek score 66.80
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
SSC
length_bp
576
variable_sites
50
pi
0.0385
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.91
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 GGGTTACCTGCTCTTAACGG ATATGTAGGGGGAAGTCGCA 55.0 50.0 58.0 57.9 792-841 bp 0.8 80.1
trnN-GUU-ndhF_p2 GGGTTACCTGCTCTTAACGG TATGTAGGGGGAAGTCGCAT 55.0 50.0 58.0 57.9 791-840 bp 0.8 80.1
trnN-GUU-ndhF_p3 GGGTTACCTGCTCTTAACGG GGGGAAGTCGCATCTCTTTT 55.0 50.0 58.0 57.9 784-833 bp 0.8 80.0
trnN-GUU-ndhF_p4 GGCAGAATAGCAGAGCAAGT ATATGTAGGGGGAAGTCGCA 50.0 50.0 58.0 57.9 1089-1138 bp 0.8 78.6
trnN-GUU-ndhF_p5 GGCAGAATAGCAGAGCAAGT TATGTAGGGGGAAGTCGCAT 50.0 50.0 58.0 57.9 1088-1137 bp 0.8 78.6