Hotspot

trnI-CAU-ycf2

IRb · 95636-95963 · 328 bp

Back to Durio

Pi 0.044
MarkerSeek score 68.60
Primer available yes
Label trnI-CAU-ycf2

Feature Metadata

label_name
trnI-CAU-ycf2
feature_type
igs
region
IRb
length_bp
328
variable_sites
21
pi
0.044
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.89
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC GTTGAACCCTGTGAATTGCG 50.0 50.0 58.0 57.9 509-644 bp 1.0 86.5
trnI-CAU-ycf2_p2 GTTGGGCGCTTTAACCATTC ACTACACCCCTGATCGTGAA 50.0 50.0 58.0 58.1 542-677 bp 1.0 86.2
trnI-CAU-ycf2_p3 GTTGGGCGCTTTAACCATTC TCCAGGAAACAGCTCTCAGA 50.0 50.0 58.0 58.0 767-902 bp 1.0 86.1
trnI-CAU-ycf2_p4 GTTGGGCGCTTTAACCATTC CCGGGTCCAATGGAATTCAT 50.0 50.0 58.0 57.9 659-794 bp 1.0 85.9
trnI-CAU-ycf2_p5 GTTGGGCGCTTTAACCATTC AATCATGGTCCTTGCGGATC 50.0 50.0 58.0 58.0 1000-1135 bp 1.0 85.6