Hotspot

psbZ-trnG-GCC

LSC · 39135-40147 · 1013 bp

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Pi 0.0176
MarkerSeek score 68.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
1013
variable_sites
34
pi
0.0176
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 1114-1216 bp 1.0 83.4
psbZ-trnG-GCC_p2 GCTTCTCCTGATGGTTGGTT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 1112-1214 bp 1.0 83.3
psbZ-trnG-GCC_p3 CTCCTGATGGTTGGTTGAGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.1 57.8 1108-1210 bp 1.0 78.1
psbZ-trnG-GCC_p4 TGCTTCTCCTGATGGTTGGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.9 57.8 1113-1215 bp 1.0 77.5
psbZ-trnG-GCC_p5 TCTCCTGATGGTTGGTTGAG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.8 57.8 1109-1211 bp 1.0 75.7