Hotspot

trnH-GUG-psbA

LSC · 483-1293 · 811 bp

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Pi 0.0206
MarkerSeek score 70.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
811
variable_sites
37
pi
0.0206
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 878-987 bp 1.0 85.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 892-1001 bp 1.0 85.3
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 891-1000 bp 1.0 85.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 893-1002 bp 1.0 85.2
trnH-GUG-psbA_p5 ATGTATATGGGCGAACGACG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 940-1912 bp 1.0 84.5