Hotspot

orf188

SSC · 124000-124563 · 564 bp

Back to Dunalia

Pi 0.00532

MarkerSeek score 61.10

Primer available yes

Label orf188

Feature Metadata

label_name: orf188
feature_type: gene
region: SSC
length_bp: 564
variable_sites: 3
pi: 0.00532
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf188_p1	AGTTCATCAACTCAAATGCTGA	TGGGAGAAGTTGATCGTTGA	36.4	45.0	56.3	56.5	692 bp	1.0	40.3
orf188_p2	AGTTCATCAACTCAAATGCTGA	TGGGAGAAGTTGATCGTTGAA	36.4	42.9	56.3	57.2	692 bp	1.0	40.3
orf188_p3	TCATCAACTCAAATGCTGAAGA	TGGGAGAAGTTGATCGTTGA	36.4	45.0	56.0	56.5	689 bp	1.0	40.3
orf188_p4	GTTCATCAACTCAAATGCTGAAGA	TGGGAGAAGTTGATCGTTGA	37.5	45.0	58.0	56.5	691 bp	1.0	40.3
orf188_p5	AGTTCATCAACTCAAATGCTGAAG	TGGGAGAAGTTGATCGTTGA	37.5	45.0	58.0	56.5	692 bp	1.0	40.3