Hotspot

ndhF-rpl32

SSC · 115009-115786 · 778 bp

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Pi 0.00386

MarkerSeek score 61.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 778
variable_sites: 3
pi: 0.00386
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAATAAGTCCCGCGCCTATT	TGCTACCCAATATCCCTTCCT	50.0	47.6	57.8	57.9	944-951 bp	1.0	70.3
ndhF-rpl32_p2	CGGCGAAAACTTTTTGTTGC	TGCTACCCAATATCCCTTCCT	45.0	47.6	57.7	57.9	975-982 bp	1.0	68.9
ndhF-rpl32_p3	GCGAAAACTTTTTGTTGCCG	TGCTACCCAATATCCCTTCCT	45.0	47.6	57.7	57.9	973-980 bp	1.0	68.9
ndhF-rpl32_p4	GTCCCGCGCCTATTAACATA	TGCTACCCAATATCCCTTCCT	50.0	47.6	57.6	57.9	938-945 bp	1.0	68.1
ndhF-rpl32_p5	GAATAAGTCCCGCGCCTATT	TGATGTCGAAGTGCGTTTTT	50.0	40.0	57.8	56.6	893-900 bp	1.0	67.7