Hotspot

trnL-UAA-trnF-GAA

LSC · 49867-50456 · 590 bp

Back to Dunalia

Pi 0.00339

MarkerSeek score 54.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 2
pi: 0.00339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	739 bp	1.0	78.3
trnL-UAA-trnF-GAA_p2	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	45.0	50.0	57.8	58.1	754 bp	1.0	77.9
trnL-UAA-trnF-GAA_p3	ACATGTCAATACCGGCAACA	AATCATGTGCCAGGAACCAG	45.0	50.0	57.8	58.2	760 bp	1.0	77.4
trnL-UAA-trnF-GAA_p4	ACATGTCAATACCGGCAACA	CATGTGCCAGGAACCAGATT	45.0	50.0	57.8	58.2	757 bp	1.0	77.4
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	680 bp	1.0	76.0