Hotspot

trnK-UUU-rps16

LSC · 4451-5146 · 696 bp

Back to Dunalia

Pi 0.00287
MarkerSeek score 60.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
696
variable_sites
2
pi
0.00287
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 828 bp 1.0 75.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 827 bp 1.0 75.1
trnK-UUU-rps16_p3 TCAGTCGTGGTCTTCCAAAC TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 913 bp 1.0 74.5
trnK-UUU-rps16_p4 CAGTCGTGGTCTTCCAAACT TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 912 bp 1.0 74.5
trnK-UUU-rps16_p5 AAAAGACACTAGCCGCACTT TTGAAAAAGGGGCTCAACCT 45.0 45.0 57.7 57.5 849 bp 1.0 74.4