Hotspot

ccsA

SSC · 111913-112872 · 960 bp

Back to Duhaldea

Pi 0.0146

MarkerSeek score 55.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 14
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	AGAACCGCGTGAATCACTAC	50.0	50.0	58.0	58.0	1253-1254 bp	1.0	80.8
ccsA_p2	TGAAATCGGTAGACACGCTG	AGAACCGCGTGAATCACTAC	50.0	50.0	58.0	58.0	1252-1253 bp	1.0	80.8
ccsA_p3	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1542-1545 bp	1.0	80.5
ccsA_p4	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1541-1544 bp	1.0	80.5
ccsA_p5	GTGAAATCGGTAGACACGCT	GCATTACCGGGTATGAGTGG	50.0	55.0	58.0	58.1	1743-1746 bp	1.0	79.9