Hotspot

ndhF-rpl32

SSC · 109945-110667 · 723 bp

Back to Duhaldea

Pi 0.0115

MarkerSeek score 69.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 723
variable_sites: 8
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	50.0	50.0	58.0	57.6	903-1144 bp	1.0	79.6
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	901-1142 bp	1.0	79.5
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	902-1143 bp	1.0	79.5
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	920-1161 bp	1.0	79.4
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	918-1159 bp	1.0	79.3