Hotspot

rps19-rpl2

IRb · 82721-82790 · 70 bp

Back to Duhaldea

Pi 0.0294

MarkerSeek score 72.10

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 70
variable_sites: 2
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGCCCGAGACCAGGTTATTA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.1	58.1	208-212 bp	1.0	79.7
rps19-rpl2_p2	TGCCCGAGACCAGGTTATTA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	300-304 bp	1.0	79.6
rps19-rpl2_p3	TGCCCGAGACCAGGTTATTA	CACAACCCCTTGGGGTTATC	50.0	55.0	58.1	58.2	275-279 bp	1.0	78.9
rps19-rpl2_p4	TGCCCGAGACCAGGTTATTA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.1	57.8	266-270 bp	1.0	78.4
rps19-rpl2_p5	TGCCCGAGACCAGGTTATTA	ATTTGATTCTTCGTCGCCGT	50.0	45.0	58.1	58.3	210-214 bp	1.0	78.2