Hotspot

psbH-petB

LSC · 73263-73388 · 126 bp

Back to Duhaldea

Pi 0.024

MarkerSeek score 67.00

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 3
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	CTTTGATGGGTGTCGCAATG	TATCCGACGAAGGAGAACCA	50.0	50.0	57.7	57.9	316-317 bp	1.0	77.8
psbH-petB_p2	CAATGGCCCTATTTGCGGTA	TATCCGACGAAGGAGAACCA	50.0	50.0	58.3	57.9	301-302 bp	1.0	77.5
psbH-petB_p3	TGGCCCTATTTGCGGTATTT	TATCCGACGAAGGAGAACCA	45.0	50.0	57.6	57.9	298-299 bp	1.0	76.4
psbH-petB_p4	CTTTGATGGGTGTCGCAATG	ATCCGACGAAGGAGAACCAT	50.0	50.0	57.7	58.5	315-316 bp	1.0	74.8
psbH-petB_p5	ATGGCCCTATTTGCGGTATT	TATCCGACGAAGGAGAACCA	45.0	50.0	57.3	57.9	299-300 bp	1.0	74.6