Hotspot

psbT-psbN

LSC · 72735-72806 · 72 bp

Back to Duhaldea

Pi 0.0417

MarkerSeek score 65.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 3
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TAGAGAACCGCCGAAAGTTC	TCGAGGAACATGGGGACTAA	50.0	50.0	57.6	57.8	126-127 bp	1.0	77.1
psbT-psbN_p2	TAGAGAACCGCCGAAAGTTC	TTCGAGGAACATGGGGACTA	50.0	50.0	57.6	57.8	127-128 bp	1.0	77.1
psbT-psbN_p3	TAGAGAACCGCCGAAAGTTC	CATTCGAGGAACATGGGGAC	50.0	55.0	57.6	58.3	129-130 bp	1.0	76.3
psbT-psbN_p4	TAGAGAACCGCCGAAAGTTC	GGGTACGCCTTATATACCGC	50.0	55.0	57.6	57.7	181-182 bp	1.0	76.2
psbT-psbN_p5	CCGCCGAAAGTTCCAACTAA	TCGAGGAACATGGGGACTAA	50.0	50.0	58.5	57.8	119-120 bp	1.0	76.2