Hotspot

ycf4-cemA

LSC · 59263-59716 · 454 bp

Back to Duhaldea

Pi 0.0114

MarkerSeek score 65.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 5
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	AGAGAGAAATCCACCAGGGT	50.0	50.0	58.0	57.6	633-637 bp	1.0	79.7
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	AGAGAGAAATCCACCAGGGT	45.0	50.0	58.1	57.6	565-569 bp	1.0	79.5
ycf4-cemA_p3	CTCCACGGGAAATGGAACAA	AGAGAGAAATCCACCAGGGT	50.0	50.0	58.1	57.6	597-601 bp	1.0	79.4
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	AGAGAGAAATCCACCAGGGT	45.0	50.0	57.8	57.6	579-583 bp	1.0	78.8
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	AGAGAGAAATCCACCAGGGT	45.0	50.0	57.8	57.6	577-581 bp	1.0	78.5