Hotspot

ndhC-trnV-UAC

LSC · 49927-50285 · 359 bp

Back to Duhaldea

Pi 0.0357
MarkerSeek score 68.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
359
variable_sites
12
pi
0.0357
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.9 57.8 392-415 bp 1.0 46.4
ndhC-trnV-UAC_p2 GACTTGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.2 57.8 424-447 bp 1.0 46.2
ndhC-trnV-UAC_p3 ACTTGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.2 57.8 423-446 bp 1.0 46.2
ndhC-trnV-UAC_p4 AGACTTGATATTATCAGAAATGCCC AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.0 57.8 425-448 bp 1.0 46.2
ndhC-trnV-UAC_p5 GACTTGATATTATCAGAAATGCCCA GAAGGTCTACGGTTCGAGTC 36.0 55.0 57.2 57.5 423-446 bp 1.0 46.2