Hotspot

psaA-ycf3

LSC · 41418-42140 · 723 bp

Back to Duhaldea

Pi 0.00979

MarkerSeek score 65.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 7
pi: 0.00979
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	913-915 bp	1.0	77.4
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	915-917 bp	1.0	77.4
psaA-ycf3_p3	CCCATGCCTCGAATGAAGTT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.2	58.3	894-896 bp	1.0	77.3
psaA-ycf3_p4	CCCATGCCTCGAATGAAGTT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.2	58.3	896-898 bp	1.0	77.3
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	914-916 bp	1.0	75.6