Hotspot

psbK-psbI

LSC · 7801-8203 · 403 bp

Back to Duhaldea

Pi 0.0226

MarkerSeek score 64.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 9
pi: 0.0226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	540-546 bp	1.0	74.1
psbK-psbI_p2	TATGCCAGTCATACCCCTGT	TCTTCACGTCCAGGATTACG	50.0	50.0	57.8	57.1	580-586 bp	1.0	72.9
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	537-543 bp	1.0	72.1
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	538-544 bp	1.0	72.1
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	539-545 bp	1.0	72.1