Hotspot

rps16-trnQ-UUG

LSC · 6309-7192 · 884 bp

Back to Duhaldea

Pi 0.0193

MarkerSeek score 56.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 17
pi: 0.0193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GATCGTTGCCCCTGAGAAAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1146-1230 bp	1.0	80.7
rps16-trnQ-UUG_p2	GATCGTTGCCCCTGAGAAAT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1720-1805 bp	1.0	80.4
rps16-trnQ-UUG_p3	AGCGGTCTTTCCTTGTTCTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1445-1525 bp	1.0	80.1
rps16-trnQ-UUG_p4	AGCGGTCTTTCCTTGTTCTG	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.1	57.9	2019-2100 bp	1.0	79.9
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.1	57.9	1531-1621 bp	1.0	79.5