Hotspot

trnK-UUU-rps16

LSC · 4375-5184 · 810 bp

Back to Duhaldea

Pi 0.01
MarkerSeek score 65.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
810
variable_sites
8
pi
0.01
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1007-1037 bp 1.0 78.3
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1006-1036 bp 1.0 78.3
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 946-976 bp 1.0 77.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 945-975 bp 1.0 77.6
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1031-1061 bp 1.0 77.0