Hotspot

trnH-GUG-psbA

LSC · 83-471 · 389 bp

Back to Duhaldea

Pi 0.0289

MarkerSeek score 58.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 11
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	55.0	55.0	58.1	58.0	1072-1080 bp	1.0	80.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	1078-1086 bp	1.0	80.4
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	55.0	55.0	58.1	58.0	1073-1081 bp	1.0	80.4
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	55.0	55.0	58.1	58.0	1074-1082 bp	1.0	80.4
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	50.0	55.0	57.9	58.0	1086-1094 bp	1.0	80.3