Hotspot

rps16

LSC · 4807-5857 · 1051 bp

Back to Dudleya

Pi 0.00127

MarkerSeek score 59.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1051
variable_sites: 2
pi: 0.00127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCGAATCATGCCCACACTTT	GCGGACCAAACCAATGACTA	50.0	50.0	58.8	58.2	1124-1125 bp	1.0	72.2
rps16_p2	TAGATCCGAATCATGCCCAC	GCGGACCAAACCAATGACTA	50.0	50.0	57.2	58.2	1129-1130 bp	1.0	72.1
rps16_p3	CCGAATCATGCCCACACTTT	TATATACGGAGTGCGGACCA	50.0	50.0	58.8	57.7	1136-1137 bp	1.0	71.5
rps16_p4	TAGATCCGAATCATGCCCAC	TATATACGGAGTGCGGACCA	50.0	50.0	57.2	57.7	1141-1142 bp	1.0	71.4
rps16_p5	CGAATCATGCCCACACTTTT	GCGGACCAAACCAATGACTA	45.0	50.0	57.0	58.2	1123-1124 bp	1.0	70.7