Hotspot

ndhA

SSC · 116761-118843 · 2083 bp

Back to Dudleya

Pi 0.0016

MarkerSeek score 58.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2083
variable_sites: 5
pi: 0.0016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTAAGTAGAGACTCGCGTGG	CCCGCCGGGTTTTATCAATT	55.0	50.0	57.3	58.9	2222-2223 bp	1.0	67.2
ndhA_p2	CTAAGTAGAGACTCGCGTGG	CCGCCGGGTTTTATCAATTT	55.0	45.0	57.3	57.0	2221-2222 bp	1.0	66.7
ndhA_p3	CTAAGTAGAGACTCGCGTGG	GCCGGGTTTTATCAATTTGCA	55.0	42.9	57.3	58.0	2219-2220 bp	1.0	66.0
ndhA_p4	CTAAGTAGAGACTCGCGTGG	CCCGCCGGGTTTTATCAAT	55.0	52.6	57.3	58.2	2222-2223 bp	1.0	64.7
ndhA_p5	ACCCAGTTACCATAGGGAACA	CCCGCCGGGTTTTATCAATT	47.6	50.0	58.1	58.9	2301-2302 bp	1.0	64.5