Hotspot

matK

LSC · 1911-3413 · 1503 bp

Back to Dudleya

Pi 0.0031
MarkerSeek score 60.70
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1503
variable_sites
7
pi
0.0031
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 CCTTAGGGGATTCTAAGAACA CCTTGTTTTGACTGTATCGCA 42.9 42.9 54.0 56.8 1709 bp 1.0 40.3
matK_p2 CCTTAGGGGATTCTAAGAACA ACCTTGTTTTGACTGTATCGC 42.9 42.9 54.0 56.8 1710 bp 1.0 40.3
matK_p3 CCTTAGGGGATTCTAAGAACA CCTTGTTTTGACTGTATCGCAC 42.9 45.5 54.0 58.2 1709 bp 1.0 40.3
matK_p4 CCTTAGGGGATTCTAAGAACA ACCTTGTTTTGACTGTATCGCA 42.9 40.9 54.0 58.5 1710 bp 1.0 40.3
matK_p5 CCTTAGGGGATTCTAAGAACAT CCTTGTTTTGACTGTATCGCA 40.9 42.9 54.7 56.8 1709 bp 1.0 40.3