Hotspot

cemA

LSC · 59694-60383 · 690 bp

Back to Dudleya

Pi 0.000966

MarkerSeek score 39.80

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 1
pi: 0.000966
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	AGAACTCACGCTTGATCGAA	AAAATTACAGTGGACCGTTT	45.0	35.0	57.3	53.3	793 bp	1.0	40.1
cemA_p2	AGAACTCACGCTTGATCGAA	AAATTACAGTGGACCGTTTT	45.0	35.0	57.3	53.3	792 bp	1.0	40.1
cemA_p3	AGAACTCACGCTTGATCGAA	AAAATTACAGTGGACCGTTTT	45.0	33.3	57.3	54.2	793 bp	1.0	40.1
cemA_p4	AGAACTCACGCTTGATCGAA	TGATTAAAATTACAGTGGACCGT	45.0	34.8	57.3	56.2	798 bp	1.0	40.1
cemA_p5	AGAACTCACGCTTGATCGAA	ACCCCTAGATTGATAGAACTAGG	45.0	43.5	57.3	56.1	876 bp	1.0	40.1