Hotspot

accD

LSC · 56159-57595 · 1437 bp

Back to Dudleya

Pi 0.000928

MarkerSeek score 45.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1437
variable_sites: 2
pi: 0.000928
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCTTTTCATTCCGTGTTGCC	GTTCCTAGAGGCAGGCTTTC	50.0	55.0	57.9	58.0	1961-1962 bp	1.0	79.5
accD_p2	CTTTTCATTCCGTGTTGCCC	GTTCCTAGAGGCAGGCTTTC	50.0	55.0	57.9	58.0	1960-1961 bp	1.0	79.5
accD_p3	CCTTTTCATTCCGTGTTGCC	TTCCTAGAGGCAGGCTTTCT	50.0	50.0	57.9	58.0	1960-1961 bp	1.0	79.4
accD_p4	CTTTTCATTCCGTGTTGCCC	TTCCTAGAGGCAGGCTTTCT	50.0	50.0	57.9	58.0	1959-1960 bp	1.0	79.4
accD_p5	CCCCGATCCTCCAAAACAAA	GTTCCTAGAGGCAGGCTTTC	50.0	55.0	58.1	58.0	1782-1783 bp	1.0	79.4