Hotspot

psbB-psbT

LSC · 72039-72217 · 179 bp

Back to Dudleya

Pi 0.00372

MarkerSeek score 40.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 1
pi: 0.00372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	CCGACGACAAGAAAACAAGC	AATTTTCGGCGGTTCTCGAA	50.0	45.0	58.0	58.5	293 bp	1.0	76.2
psbB-psbT_p2	CGACAAGAAAACAAGCCGTC	AATTTTCGGCGGTTCTCGAA	50.0	45.0	58.0	58.5	289 bp	1.0	76.2
psbB-psbT_p3	CGACGACAAGAAAACAAGCC	AATTTTCGGCGGTTCTCGAA	50.0	45.0	58.0	58.5	292 bp	1.0	76.2
psbB-psbT_p4	GACGACAAGAAAACAAGCCG	AATTTTCGGCGGTTCTCGAA	50.0	45.0	58.0	58.5	291 bp	1.0	76.2
psbB-psbT_p5	TGCGAGAACTCTGTTTCGAG	AATTTTCGGCGGTTCTCGAA	50.0	45.0	57.9	58.5	384 bp	1.0	75.9