Hotspot

pafII-cemA

LSC · 59080-59693 · 614 bp

Back to Dudleya

Pi 0.00635

MarkerSeek score 64.00

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 5
pi: 0.00635
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	TGACTCCACGCGAAATTGAA	AAGAGAGATCAACCAGGGCA	45.0	50.0	58.1	58.3	672-763 bp	1.0	80.8
pafII-cemA_p2	TGACTCCACGCGAAATTGAA	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.1	58.3	671-762 bp	1.0	80.8
pafII-cemA_p3	TTGACTCCACGCGAAATTGA	AAGAGAGATCAACCAGGGCA	45.0	50.0	58.1	58.3	673-764 bp	1.0	80.8
pafII-cemA_p4	TTGACTCCACGCGAAATTGA	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.1	58.3	672-763 bp	1.0	80.8
pafII-cemA_p5	AGAATTTGACTCCACGCGAA	AGAGAGATCAACCAGGGCAA	45.0	50.0	57.8	58.3	677-768 bp	1.0	80.5