Hotspot

trnF-GAA-ndhJ

LSC · 47296-47872 · 577 bp

Back to Dudleya

Pi 0.00347

MarkerSeek score 60.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 3
pi: 0.00347
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCCCGCTTGAAACGTATC	55.0	50.0	58.0	58.1	751-762 bp	1.0	79.9
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	719-730 bp	1.0	79.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCCCCGCTTGAAACGTATC	50.0	50.0	58.1	58.1	715-726 bp	1.0	79.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	683-694 bp	1.0	79.2
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	ATCCCCGCTTGAAACGTATC	55.0	50.0	57.9	58.1	739-750 bp	1.0	78.8