Hotspot

psbZ-trnG-UCC

LSC · 35807-36476 · 670 bp

Back to Dudleya

Pi 0.00199

MarkerSeek score 60.00

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 2
pi: 0.00199
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CGTTGTATTTGCTTCGCCTG	TACCATTCGACCATATCCGC	50.0	50.0	58.1	57.3	808 bp	1.0	73.4
psbZ-trnG-UCC_p2	CGTTGTATTTGCTTCGCCTG	TTACCATTCGACCATATCCGC	50.0	47.6	58.1	58.0	809 bp	1.0	71.0
psbZ-trnG-UCC_p3	TATTTGCTTCGCCTGATGGT	TACCATTCGACCATATCCGC	45.0	50.0	57.6	57.3	803 bp	1.0	71.0
psbZ-trnG-UCC_p4	ATTTGCTTCGCCTGATGGTT	TACCATTCGACCATATCCGC	45.0	50.0	58.5	57.3	802 bp	1.0	70.7
psbZ-trnG-UCC_p5	GCCTGATGGTTGGTCAAGTA	TACCATTCGACCATATCCGC	50.0	50.0	57.5	57.3	793 bp	1.0	70.4