Hotspot

rpoB-trnC-GCA

LSC · 26853-27975 · 1123 bp

Back to Dudleya

Pi 0.00297

MarkerSeek score 48.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 5
pi: 0.00297
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1211-1213 bp	1.0	79.5
rpoB-trnC-GCA_p2	GTGGACCTTCCCTCATTTCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1217-1219 bp	1.0	78.6
rpoB-trnC-GCA_p3	TGGACCTTCCCTCATTTCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1216-1218 bp	1.0	77.7
rpoB-trnC-GCA_p4	CTTCCCTCATTTCCATCCCG	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	58.4	1193-1195 bp	1.0	77.3
rpoB-trnC-GCA_p5	AGGTATTGTGGACCTTCCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.6	58.1	1224-1226 bp	1.0	76.8