Hotspot

atpF-atpH

LSC · 13296-13684 · 389 bp

Back to Dudleya

Pi 0.00343

MarkerSeek score 44.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 2
pi: 0.00343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGAGGCGGAGGGAAAAATAA	50.0	50.0	57.9	58.0	576 bp	1.0	79.1
atpF-atpH_p2	CGGATGACCAGTAACCCAAA	CGAGGCGGAGGGAAAAATAA	50.0	50.0	57.5	58.0	556 bp	1.0	76.2
atpF-atpH_p3	CGGATGACCAGTAACCCAAAT	CGAGGCGGAGGGAAAAATAA	47.6	50.0	58.0	58.0	556 bp	1.0	72.0
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATAAGAGGT	50.0	47.6	57.9	58.0	572 bp	1.0	71.3
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GCTTAGTCTGGCTTTCATGGA	50.0	47.6	57.9	58.0	543 bp	1.0	71.3