Hotspot

trnH-GUG-psbA

LSC · 73-344 · 272 bp

Back to Dudleya

Pi 0.0172

MarkerSeek score 52.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 272
variable_sites: 7
pi: 0.0172
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	AATCCACTTGGCTACATCCG	TGAAGTCCCATCTTTAAATGG	50.0	38.1	57.7	53.5	316-319 bp	1.0	42.8
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TGAAGTCCCATCTTTAAATGG	50.0	38.1	57.9	53.5	323-326 bp	1.0	42.7
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	TGAAGTCCCATCTTTAAATGG	50.0	38.1	58.2	53.5	324-327 bp	1.0	42.7
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TTGAAGTCCCATCTTTAAATGG	50.0	36.4	57.9	54.3	324-327 bp	1.0	42.7
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TTGAAGTCCCATCTTTAAATGG	50.0	36.4	58.2	54.3	325-328 bp	1.0	42.7