Hotspot

ndhD-psaC

Genome · 96535-96673 · 139 bp

Back to Drypetes

Pi 0.0489

MarkerSeek score 78.30

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: Genome
length_bp: 139
variable_sites: 15
pi: 0.0489
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCGCGGATATCGGTAAAACT	AAACAACTCGAAGCATGGGT	50.0	45.0	57.9	58.0	222-228 bp	1.0	85.1
ndhD-psaC_p2	CCGCGGATATCGGTAAAACT	GGCATGAAACAACTCGAAGC	50.0	50.0	57.9	58.0	228-234 bp	1.0	84.9
ndhD-psaC_p3	CCGCGGATATCGGTAAAACT	AGAGGACTGTGTCGGTTGTA	50.0	50.0	57.9	58.0	302-308 bp	1.0	83.5
ndhD-psaC_p4	CCGCGGATATCGGTAAAACT	AAGAGATGTGAATCCGCCTG	50.0	50.0	57.9	57.7	283-289 bp	1.0	81.3
ndhD-psaC_p5	CCGCGGATATCGGTAAAACT	GAGATGTGAATCCGCCTGTC	50.0	55.0	57.9	58.4	281-287 bp	1.0	80.6