Hotspot

rpl14-rps8

Genome · 12392-12601 · 210 bp

Back to Drypetes

Pi 0.0543

MarkerSeek score 81.50

Primer available yes

Label rpl14-rps8

Feature Metadata

label_name: rpl14-rps8
feature_type: igs
region: Genome
length_bp: 210
variable_sites: 21
pi: 0.0543
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps8_p1	ATTTTTGGTGCGATCCTCGA	AGCCATGTCAGCATTTCGTA	45.0	45.0	57.9	57.6	338-377 bp	1.0	82.4
rpl14-rps8_p2	ATTTTTGGTGCGATCCTCGA	TAGCCATGTCAGCATTTCGT	45.0	45.0	57.9	57.6	339-378 bp	1.0	82.4
rpl14-rps8_p3	TTTTTGGTGCGATCCTCGAG	AGCCATGTCAGCATTTCGTA	50.0	45.0	58.6	57.6	337-376 bp	1.0	78.8
rpl14-rps8_p4	TTTTTGGTGCGATCCTCGAG	TAGCCATGTCAGCATTTCGT	50.0	45.0	58.6	57.6	338-377 bp	1.0	78.8
rpl14-rps8_p5	ATTTTTGGTGCGATCCTCGA	AGCCATGTCAGCATTTCGTAT	45.0	42.9	57.9	58.1	338-377 bp	1.0	77.0