Hotspot

trnQ-UUG-psbK

Genome · 5408-6122 · 715 bp

Back to Drypetes

Pi 0.0551

MarkerSeek score 77.40

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: Genome
length_bp: 715
variable_sites: 75
pi: 0.0551
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	ATAGGTATGCTCTGGGACGG	AAGAGGACAGGGATTACGGG	55.0	55.0	58.4	58.5	595-1015 bp	1.0	84.5
trnQ-UUG-psbK_p2	ATAGGTATGCTCTGGGACGG	CAGGGATTACGGGCATAACA	55.0	50.0	58.4	57.4	588-1008 bp	1.0	83.5
trnQ-UUG-psbK_p3	ATAGGTATGCTCTGGGACGG	ACAGGGATTACGGGCATAAC	55.0	50.0	58.4	57.4	589-1009 bp	1.0	83.5
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	AAGAGGACAGGGATTACGGG	50.0	55.0	58.7	58.5	550-970 bp	1.0	82.3
trnQ-UUG-psbK_p5	ATAGGTATGCTCTGGGACGG	TAGCAGCTTGCCAAACAAAG	55.0	45.0	58.4	57.2	627-1047 bp	1.0	81.9