Hotspot

trnK-UUU-trnQ-UUG

Genome · 4505-5335 · 831 bp

Back to Drypetes

Pi 0.0737

MarkerSeek score 83.30

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 831
variable_sites: 119
pi: 0.0737
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	GGACGAATCCGCTTCTTCAT	GTTCGAAACCTTCCGTCCC	50.0	57.9	58.1	58.5	791-983 bp	1.0	79.8
trnK-UUU-trnQ-UUG_p2	CCGATGGTATGGACGAATCC	GTTCGAAACCTTCCGTCCC	55.0	57.9	57.9	58.5	801-993 bp	1.0	79.7
trnK-UUU-trnQ-UUG_p3	AAAGCCGAGTACTCTACCGT	GTTCGAAACCTTCCGTCCC	50.0	57.9	58.2	58.5	740-932 bp	1.0	78.9
trnK-UUU-trnQ-UUG_p4	AAGCCGAGTACTCTACCGTT	GTTCGAAACCTTCCGTCCC	50.0	57.9	58.2	58.5	739-931 bp	1.0	78.9
trnK-UUU-trnQ-UUG_p5	GTTAGCCGCACTTAAAAGCC	GTTCGAAACCTTCCGTCCC	50.0	57.9	57.7	58.5	754-946 bp	1.0	78.2