Hotspot

petB

Genome · 9935-11385 · 1451 bp

Back to Dryopteris

Pi 0.0226

MarkerSeek score 60.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 1451
variable_sites: 61
pi: 0.0226
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GTCACACACTCCCGTAATCC	AATTGAGCAGGCTCTATGGC	55.0	50.0	58.0	58.0	2006-2014 bp	1.0	83.9
petB_p2	CAACATGGCTTCTCATCCGA	AATTGAGCAGGCTCTATGGC	50.0	50.0	58.0	58.0	2493-2501 bp	1.0	83.3
petB_p3	GTTTCGCAACATGGCTTCTC	AATTGAGCAGGCTCTATGGC	50.0	50.0	58.0	58.0	2499-2507 bp	1.0	83.3
petB_p4	AGAAAGGTCGGAAAGGGAGA	AATTGAGCAGGCTCTATGGC	50.0	50.0	58.0	58.0	2095 bp	0.25	49.6
petB_p5	GTCACACACTCCCGTAATCC	TCGAAGGAAGGTCCATCAGT	55.0	50.0	58.0	58.1	2244 bp	0.25	49.5