Hotspot

ndhB

Genome · 80988-83296 · 2309 bp

Back to Dryopteris

Pi 0.0173

MarkerSeek score 57.10

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 2309
variable_sites: 77
pi: 0.0173
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	AGCGGAAGGTTATCCACTCT	AAGTTCGCGTCACAAGAAGT	50.0	45.0	58.1	58.1	2352-2376 bp	0.75	71.6
ndhB_p2	GCGGAAGGTTATCCACTCTC	AAGTTCGCGTCACAAGAAGT	55.0	45.0	57.8	58.1	2351-2375 bp	0.75	70.9
ndhB_p3	TGTAGCGGAAGGTTATCCAC	AAGTTCGCGTCACAAGAAGT	50.0	45.0	57.0	58.1	2355-2379 bp	0.75	64.9
ndhB_p4	TAGCGGAAGGTTATCCACTCT	AAGTTCGCGTCACAAGAAGT	47.6	45.0	58.0	58.1	2353-2377 bp	0.75	64.5
ndhB_p5	AGCGGAAGGTTATCCACTCT	AAAGTTCGCGTCACAAGAAG	50.0	45.0	58.1	57.0	2353-2377 bp	0.75	64.0