Hotspot

trnP-GGG-rpl32

Genome · 122727-123345 · 619 bp

Back to Dryopteris

Pi 0.0493
MarkerSeek score 79.40
Primer available yes
Label trnP-GGG-rpl32

Feature Metadata

label_name
trnP-GGG-rpl32
feature_type
igs
region
Genome
length_bp
619
variable_sites
59
pi
0.0493
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-rpl32_p1 GAGGTCACAGGTTCGAATCC CGTCATGGATCTGGCATCAA 55.0 50.0 58.0 58.0 1217-1226 bp 0.75 74.7
trnP-GGG-rpl32_p2 GGAGGTCACAGGTTCGAATC CGTCATGGATCTGGCATCAA 55.0 50.0 58.0 58.0 1218-1227 bp 0.75 74.7
trnP-GGG-rpl32_p3 CTGGCACTGCTTCCTAAGAG CGTCATGGATCTGGCATCAA 55.0 50.0 58.0 58.0 1596-1599 bp 0.75 74.4
trnP-GGG-rpl32_p4 GAGGTCACAGGTTCGAATCC TTTCGGCTATCCATGGTTGG 55.0 50.0 58.0 57.9 1203 bp 0.25 49.5
trnP-GGG-rpl32_p5 GGAGGTCACAGGTTCGAATC TTTCGGCTATCCATGGTTGG 55.0 50.0 58.0 57.9 1204 bp 0.25 49.5