Hotspot

trnL-UAG-trnP-GGG

Genome · 122373-122652 · 280 bp

Back to Dryopteris

Pi 0.0452

MarkerSeek score 73.90

Primer available yes

Label trnL-UAG-trnP-GGG

Feature Metadata

label_name: trnL-UAG-trnP-GGG
feature_type: igs
region: Genome
length_bp: 280
variable_sites: 25
pi: 0.0452
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-trnP-GGG_p1	CTCTGGCACTGCTTCCTAAG	GATTCGAACCTGTGACCTCC	55.0	55.0	58.0	58.0	394-400 bp	1.0	84.8
trnL-UAG-trnP-GGG_p2	CTCTGGCACTGCTTCCTAAG	GGATTCGAACCTGTGACCTC	55.0	55.0	58.0	58.0	395-401 bp	1.0	84.8
trnL-UAG-trnP-GGG_p3	CTGGCACTGCTTCCTAAGAG	GATTCGAACCTGTGACCTCC	55.0	55.0	58.0	58.0	392-398 bp	1.0	84.8
trnL-UAG-trnP-GGG_p4	CTGGCACTGCTTCCTAAGAG	GGATTCGAACCTGTGACCTC	55.0	55.0	58.0	58.0	393-399 bp	1.0	84.8
trnL-UAG-trnP-GGG_p5	TCCTAAGAGCAGCGTGTCTA	GATTCGAACCTGTGACCTCC	50.0	55.0	57.9	58.0	381-387 bp	1.0	84.1