Hotspot

ndhE-psaC

Genome · 118501-118824 · 324 bp

Back to Dryopteris

Pi 0.0454

MarkerSeek score 71.40

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: Genome
length_bp: 324
variable_sites: 26
pi: 0.0454
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	AGCGAAATAGAAGATCTACTCGT	ATTTTCACTGAATGTGCCAT	39.1	35.0	57.0	53.7	393-402 bp	1.0	46.3
ndhE-psaC_p2	GCGAAATAGAAGATCTACTCGT	ATTTTCACTGAATGTGCCAT	40.9	35.0	55.6	53.7	392-401 bp	1.0	46.3
ndhE-psaC_p3	TCAGCGAAATAGAAGATCTACTCG	ATTTTCACTGAATGTGCCAT	41.7	35.0	58.0	53.7	395-404 bp	1.0	46.2
ndhE-psaC_p4	CAGCGAAATAGAAGATCTACTCGT	ATTTTCACTGAATGTGCCAT	41.7	35.0	58.2	53.7	394-403 bp	1.0	46.2
ndhE-psaC_p5	CAGCGAAATAGAAGATCTACTCG	ATTTTCACTGAATGTGCCAT	43.5	35.0	56.7	53.7	394-403 bp	1.0	46.2