Hotspot

rps16-matK

Genome · 78604-79272 · 669 bp

Back to Dryopteris

Pi 0.0411

MarkerSeek score 73.30

Primer available yes

Label rps16-matK

Feature Metadata

label_name: rps16-matK
feature_type: igs
region: Genome
length_bp: 669
variable_sites: 53
pi: 0.0411
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-matK_p1	GTCCGTTACCCCGTATGTTC	ATATTGCACTACAAGCCCCG	55.0	50.0	58.1	58.0	1444-1669 bp	1.0	84.7
rps16-matK_p2	AAGGGGTAGAACGTTCCTGA	ATATTGCACTACAAGCCCCG	50.0	50.0	58.0	58.0	1607-1827 bp	0.75	75.1
rps16-matK_p3	AAGGGGTAGAACGTTCCTGA	ACTTATGCATTCGCGTCCAT	50.0	45.0	58.0	58.0	2117-2337 bp	0.75	74.5
rps16-matK_p4	CGCGGTTAAGCCTCATTAGT	ATATTGCACTACAAGCCCCG	50.0	50.0	58.1	58.0	1462-1687 bp	0.75	74.3
rps16-matK_p5	CGCGGTTAAGCCTCATTAGT	ACTTATGCATTCGCGTCCAT	50.0	45.0	58.1	58.0	1972-2197 bp	0.75	73.8