Hotspot

psbI-psbK

Genome · 74680-75191 · 512 bp

Back to Dryopteris

Pi 0.0446

MarkerSeek score 74.30

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: Genome
length_bp: 512
variable_sites: 44
pi: 0.0446
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	AGACAAATAGTTTGAGGGTAAGCA	CCGCAGTTAGCTTTCGCTA	37.5	52.6	58.1	58.0	553-563 bp	1.0	47.6
psbI-psbK_p2	TGCATAGACAAATAGTTTGAGGGT	CCGCAGTTAGCTTTCGCTA	37.5	52.6	58.2	58.0	558-568 bp	1.0	47.5
psbI-psbK_p3	AGACAAATAGTTTGAGGGTAAGCA	TCCTCCTAGCCTTTGTTTGG	37.5	50.0	58.1	57.1	577-587 bp	1.0	47.3
psbI-psbK_p4	GACAAATAGTTTGAGGGTAAGCA	TCCTCCTAGCCTTTGTTTGG	39.1	50.0	56.8	57.1	576-586 bp	1.0	47.3
psbI-psbK_p5	TGCATAGACAAATAGTTTGAGGGT	TCCTCCTAGCCTTTGTTTGG	37.5	50.0	58.2	57.1	582-592 bp	1.0	47.2