Hotspot

trnS-UGA-psbI

Genome · 74496-74568 · 73 bp

Back to Dryopteris

Pi 0.0479

MarkerSeek score 75.80

Primer available yes

Label trnS-UGA-psbI

Feature Metadata

label_name: trnS-UGA-psbI
feature_type: igs
region: Genome
length_bp: 73
variable_sites: 7
pi: 0.0479
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbI_p1	GGGTTCGAATCCCTCTCTCT	AATGATCCTGGACGAAACCC	55.0	50.0	58.2	57.6	133 bp	1.0	79.1
trnS-UGA-psbI_p2	GGACTGCTAATCCGTCGTAC	AATGATCCTGGACGAAACCC	55.0	50.0	57.9	57.6	169 bp	1.0	79.0
trnS-UGA-psbI_p3	GACTGCTAATCCGTCGTACA	AATGATCCTGGACGAAACCC	50.0	50.0	57.2	57.6	168 bp	1.0	73.3
trnS-UGA-psbI_p4	TAAGGCGTCGGACTGCTAAT	AATGATCCTGGACGAAACCC	50.0	50.0	58.9	57.6	178 bp	1.0	72.7
trnS-UGA-psbI_p5	GGACTGCTAATCCGTCGTAC	AAATGATCCTGGACGAAACCC	55.0	47.6	57.9	58.3	170 bp	1.0	72.1