Hotspot

trnP-UGG-trnW-CCA

Genome · 19542-19727 · 186 bp

Back to Dryopteris

Pi 0.0739

MarkerSeek score 77.20

Primer available yes

Label trnP-UGG-trnW-CCA

Feature Metadata

label_name: trnP-UGG-trnW-CCA
feature_type: igs
region: Genome
length_bp: 186
variable_sites: 25
pi: 0.0739
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-trnW-CCA_p1	GTTCAAATCCTGCCATCCCT	GACATCGGGTTTTGGAGACC	50.0	55.0	57.9	58.5	254-256 bp	1.0	82.9
trnP-UGG-trnW-CCA_p2	AGGTTCAAATCCTGCCATCC	GACATCGGGTTTTGGAGACC	50.0	55.0	57.9	58.5	256-258 bp	1.0	82.8
trnP-UGG-trnW-CCA_p3	GGTAGCGTGTTTGTTTTGGG	GACATCGGGTTTTGGAGACC	50.0	55.0	57.9	58.5	289-291 bp	1.0	82.0
trnP-UGG-trnW-CCA_p4	CGGTAGCGTGTTTGTTTTGG	GACATCGGGTTTTGGAGACC	50.0	55.0	58.3	58.5	290-292 bp	1.0	81.0
trnP-UGG-trnW-CCA_p5	GGTAGCGTGTTTGTTTTGGG	CGACATCGGGTTTTGGAGAC	50.0	55.0	57.9	58.9	290-292 bp	1.0	79.0