Hotspot

psaJ-trnP-UGG

Genome · 19094-19467 · 374 bp

Back to Dryopteris

Pi 0.046
MarkerSeek score 72.20
Primer available yes
Label psaJ-trnP-UGG

Feature Metadata

label_name
psaJ-trnP-UGG
feature_type
igs
region
Genome
length_bp
374
variable_sites
33
pi
0.046
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-trnP-UGG_p1 GTAGGTGTGTTACGGCGATT GAACCTGCGGCATTTTGTAC 50.0 50.0 58.0 58.0 931-944 bp 1.0 84.3
psaJ-trnP-UGG_p2 AACGTCTACGGAGAGATCGT GAACCTGCGGCATTTTGTAC 50.0 50.0 58.0 58.0 1114-1128 bp 1.0 83.7
psaJ-trnP-UGG_p3 GTAGGTGTGTTACGGCGATT GAGTGAGGAGGAAGCGAAAG 50.0 55.0 58.0 58.0 1070-1073 bp 0.5 63.0
psaJ-trnP-UGG_p4 AACGTCTACGGAGAGATCGT GAGTGAGGAGGAAGCGAAAG 50.0 55.0 58.0 58.0 1253-1257 bp 0.5 62.6
psaJ-trnP-UGG_p5 GAGGAACGTCTACGGAGAGA GAGTGAGGAGGAAGCGAAAG 55.0 55.0 58.0 58.0 1257-1261 bp 0.5 62.6